TB Profiler
This tool processes raw sequence data to infer strain type and identify known drug resistance markers.
A stand alone version of this tool is now available and can now be run on linux or mac. Installation instructions can be found on GitHub and on the bioconda page.



This tool is for Research Use Only. Data and information provided through use of this tool are not intended for medical purpose or objective and should not be used for clinical diagnosis, patient management, or human clinical trials.
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Single nucleotide polymorphisms (SNPs) in coding regions are annotated using the reference amino acid, codon number and alternative amino acid (e.g. Ser315Thr in katG). SNPs in non-coding regions (i.e. RNA genes and intergenic regions) are annotated using the reference nucleotide, gene coordinate and alternative nucleotide (e.g. A1401G in rrs or C-37A in eis promoter). Indels are annotated using the reference VCF allele, gene coordinate and alternative VCF allele (e.g. T902TA insertion in katG).
Name: 5cc551ba79bdf
Sample: 5cc551ba79bdf

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Summary
ID: 5cc551ba79bdf
Date: Sun Apr 28 08:11:18 2019
Strain: lineage4.3;lineage4.4.1;lineage4.6.1;lineage2.2.1.1
Drug-resistance: Sensitive

Drug1ResistanceSupporting Mutations (frequency)
Isoniazid
Rifampicin
Ethambutol
Pyrazinamide
Streptomycin
Ethionamide
Fluoroquinolones
Amikacin
Capreomycin
Kanamycin

Lineage2FamilyMain SpoligotypeRDS
lineage4.3Euro-American (LAM)mainly-LAMNone
lineage4.4.1Euro-American (S-type)S;TNone
lineage4.6.1Euro-American (Uganda)T2RD724
lineage2.2.1.1East-Asian (Beijing)Beijing-RD150RD105;RD207;RD181;RD150

Mutations in candidate genes:
GeneChromosome PositionMutationEstimated fraction
gyrB6319c.1080G>C1.000
gyrA8480c.1179C>T1.000
gyrA8486c.1185T>C1.000
gyrA8507c.1206C>T1.000
rpoB759571c.-236C>A1.000
rpoB759606c.-201T>C1.000
rpoB759629c.-178T>C1.000
rpoB760181c.375T>G1.000
rpoB760184c.378A>G1.000
rpoB760196c.390C>G1.000
rpoB760235c.429T>C1.000
rpoB760283c.477G>C1.000
rpoB760298c.492G>C1.000
rpoB760340c.534G>T1.000
rpoB760424c.618C>G1.000
rpoB760646c.840C>G1.000
rpoB760649c.843G>C1.000
rpoB760655c.849A>G1.000
rpoB760946c.1140A>G1.000
rpoB760982c.1176G>C1.000
rpoB760985c.1179G>C1.000
rpoB760991c.1185G>C1.000
rpoB761006c.1200C>T1.000
rpoB761015c.1209G>C1.000
rpoB761027c.1221A>G1.000
rpoB761036c.1230G>C1.000
rpoB761037c.1231T>C1.000
rpoB761057c.1251G>C1.000
rpoB761102c.1296A>G1.000
rpoB761129c.1323G>C1.000
rpoB761133c.1327T>C1.000
rpoB761150c.1344A>C0.857
rpoB761165c.1359G>C1.000
rpoB761180c.1374A>C1.000
rpoB761189c.1383T>C1.000
rpoB761195c.1389G>C1.000
rpoB761207c.1401C>T1.000
rpoB761213c.1407G>C1.000
rpoB761234c.1428G>C1.000
rpoB761249c.1443A>G1.000
rpoB761255c.1449T>G1.000
rpoB761261c.1455G>T1.000
rpoB761286c.1480C>T1.000
rpoB761289p.Ser495Ala1.000
rpoB761292p.Val496Thr1.000
rpoB761300c.1494G>C1.000
rpoB761327c.1521A>G1.000
rpoB761558c.1752C>G1.000
rpoB761579c.1773G>C1.000
rpoB761942c.2136C>T1.000
rpoB761948c.2142G>C1.000
rpoB761954c.2148C>G1.000
rpoB761999c.2193G>C1.000
rpoB762014c.2208C>T1.000
rpoB762017c.2211A>G1.000
rpoB762206c.2400C>G1.000
rpoB762212c.2406G>C1.000
rpoB762233c.2427G>C1.000
rpoB762254c.2448T>G1.000
rpoB762257c.2451C>G1.000
rpoB762260c.2454C>T1.000
rpoB762284c.2478G>C1.000
rpoB762293c.2487T>C1.000
rpoB762863c.3057T>C1.000
rpoB762929c.3123G>T1.000
rpoB762962c.3156C>T1.000
rpoB762971c.3165G>A1.000
rpoB762989c.3183G>C1.000
rpoB762995c.3189G>T1.000
rpoB763028c.3222T>C1.000
rpoB763031c.3225T>G1.000
rpoB763040c.3234C>G1.000
rpoB763070c.3264T>C1.000
rpoB763115c.3309T>C1.000
rpoB763136c.3330C>T1.000
rpoB763148c.3342G>C1.000
rpoB763158c.3352C>T1.000
rpoB763166c.3360A>G1.000
rpoC763351c.-19T>C1.000
rpoC763352c.-18T>A1.000
rpoC763402c.33C>A1.000
rpoC763411c.42T>G1.000
rpoC763414c.45T>C1.000
rpoC763594c.225C>T1.000
rpoC763603c.234C>T1.000
rpoC763660c.291T>G1.000
rpoC763675c.306C>G1.000
rpoC763696c.327T>C1.000
rpoC763702c.333C>G1.000
rpoC763717c.348T>C1.000
rpoC763723c.354G>C1.000
rpoC763732c.363C>G1.000
rpoC763741c.372C>T1.000
rpoC763744c.375G>C1.000
rpoC763765c.396T>C1.000
rpoC763774c.405G>C1.000
rpoC764140c.771C>T1.000
rpoC764257c.888G>C1.000
rpoC764665c.1296C>G1.000
rpoC764713c.1344G>C1.000
rpoC764716c.1347G>C1.000
rpoC764752c.1383G>C1.000
rpoC764764c.1395T>C1.000
rpoC764797c.1428G>C1.000
rpoC764803c.1434C>T1.000
rpoC764809c.1440C>T1.000
rpoC764815c.1446A>G1.000
rpoC764827c.1458G>C1.000
rpoC764858c.1489T>C1.000
rpoC764888c.1519T>C1.000
rpoC764893c.1524T>C1.000
rpoC764911c.1542A>G1.000
rpoC764932c.1563C>A0.947
rpoC764935c.1566T>C1.000
rpoC764948c.1579T>C1.000
rpoC764968c.1599T>C1.000
rpoC764995c.1626C>G1.000
rpoC765047c.1678T>C1.000
rpoC765055c.1686C>G1.000
rpoC765103c.1734G>T1.000
rpoC765478c.2109T>C1.000
rpoC765499c.2130C>G1.000
rpoC765508c.2139C>G1.000
rpoC765529c.2160C>T1.000
rpoC765541c.2172C>G1.000
rpoC765553c.2184C>T1.000
rpoC765556c.2187G>C1.000
rpoC765559c.2190G>C1.000
rpoC765580c.2211G>C1.000
rpoC765613p.His748Gln1.000
rpoC765658c.2289C>T1.000
rpoC765700c.2331T>C1.000
rpoC765734c.2365T>C1.000
rpoC765739c.2370G>T1.000
rpoC765751c.2382C>G1.000
rpoC765753p.Asp795Ala1.000
rpoC765772c.2403C>G1.000
rpoC765784c.2415C>G1.000
rpoC765793c.2424C>G1.000
rpoC765796c.2427C>T1.000
rpoC765826c.2457T>C1.000
rpoC765835c.2466C>T1.000
rpoC765883c.2514C>G1.000
rpoC765886c.2517C>G1.000
rpoC765892c.2523T>C1.000
rpoC765967c.2598C>T1.000
rpoC765979c.2610C>G1.000
rpoC765982c.2613C>T1.000
rpoC765994c.2625A>T1.000
rpoC766009c.2640G>C1.000
rpoC766010c.2641T>A1.000
rpoC766021c.2652G>C1.000
rpoC766027c.2658G>C1.000
rpoC766043p.Gln892Glu1.000
rpoC766054c.2685C>G1.000
rpoC766061p.Val898Ile1.000
rpoC766408c.3039C>T1.000
rpoC766426c.3057C>T1.000
rpoC766447c.3078T>C1.000
rpoC766459c.3090G>T0.923
rpoC766483c.3114G>C1.000
rpoC766484p.Val1039Ile1.000
rpoC766492c.3123T>C1.000
rpoC766495c.3126C>T1.000
rpoC766522c.3153C>G1.000
rpoC766528c.3159T>C1.000
rpoC766531c.3162G>T1.000
rpoC766540p.Asp1057Glu1.000
rpoC766549c.3180G>C1.000
rpoC766726c.3357T>C1.000
rpoC766738c.3369G>C1.000
rpoC766801c.3432C>G1.000
rpoC766804c.3435A>G1.000
rpoC766861c.3492G>C1.000
rpoC766864c.3495G>C1.000
rpoC766894c.3525T>C1.000
rpoC766895c.3526T>C1.000
rpoC766900c.3531T>C1.000
rpoC766903c.3534C>T1.000
rpoC766918c.3549C>T1.000
rpoC766933c.3564A>G1.000
rpoC766945c.3576A>G1.000
rpoC766963c.3594T>C1.000
rpoC766972c.3603G>C1.000
rpoC766996c.3627C>T1.000
rpoC767032c.3663G>T1.000
rpoC767035c.3666G>T1.000
rpoC767059c.3690T>G1.000
rpoC767062c.3693C>A1.000
rpoC767098c.3729T>C1.000
rpoC767119c.3750A>G1.000
rpoC767134c.3765C>A1.000
rpoC767158c.3789T>C0.923
rpoC767167c.3798C>G1.000
rpoC767180p.Ala1271Gln1.000
rpoC767191c.3822C>G1.000
rpsL781649c.90T>C1.000
rpsL781655c.96T>C1.000
rpsL781700c.141G>C1.000
rpsL781703c.144G>T1.000
rpsL781715c.156T>C1.000
rpsL781728c.169T>C1.000
rpsL781736c.177T>C1.000
rpsL781742c.183C>T1.000
rpsL781754c.195G>C1.000
rpsL781760c.201T>C1.000
rpsL781769c.210G>A1.000
rpsL781772c.213C>T1.000
rpsL781808c.249C>T1.000
rpsL781811c.252C>T1.000
rpsL781832c.273T>C1.000
rpsL781838c.279G>C1.000
rpsL781841c.282C>G1.000
rpsL781865c.306G>C1.000
rpsL781868c.309T>C1.000
rpsL781871c.312G>C1.000
rpsL781877c.318T>C1.000
rpsL781892c.333A>G1.000
rpsL781898c.339A>T1.000
rpsL781916c.357T>C1.000
rpsL781929p.Gly124Ser1.000
rplC800814c.6A>G1.000
rplC800817c.9A>T1.000
rrs1471923r.78t>c1.000
rrs1471925r.80t>c1.000
rrs1471932r.87a>g1.000
rrs1471934r.89a>g1.000
rrs1471969r.124t>c1.000
rrs1471970r.125g>a1.000
rrs1472028r.183a>t1.000
rrs1472029r.184c>t1.000
rrs1472030r.185g>t1.000
rrs1472031r.186g>a1.000
rrs1472034r.189t>c1.000
rrs1472044r.199g>t1.000
rrs1472053r.208agc>a1.000
rrs1472061r.216a>t1.000
rrs1472075r.230a>g1.000
rrs1472108r.263c>t1.000
rrs1472969r.1124a>g1.000
rrs1472970r.1125c>g1.000
rrs1472977r.1132g>c1.000
rrs1472978r.1133t>c1.000
rrs1473105r.1260g>a1.000
rrs1473120r.1275c>ct0.850
rrs1473123r.1278a>t0.947
rrs1473145r.1300c>t1.000
rrs1473166r.1321g>a1.000
rrs1473290r.1445c>t1.000
rrl1473668r.11c>t1.000
rrl1473756r.99g>a1.000
rrl1473758r.101g>t1.000
rrl1473806r.149c>t1.000
rrl1473877r.220g>a1.000
rrl1473898r.241c>t1.000
rrl1473899r.242a>c1.000
rrl1473938r.281g>a1.000
rrl1473939r.282c>t1.000
rrl1473943r.286g>t1.000
rrl1473986r.329t>c1.000
rrl1473995r.338g>t1.000
rrl1474001r.344c>t1.000
rrl1474009r.352a>g1.000
rrl1474012r.355c>t1.000
rrl1474044r.387c>t1.000
rrl1474054r.397t>c1.000
rrl1474093r.436g>t1.000
rrl1474099r.442g>a1.000
rrl1474103r.446a>t1.000
rrl1474140r.483c>t1.000
rrl1474151r.494c>t1.000
rrl1474181r.524c>t1.000
rrl1474249r.592g>t1.000
rrl1474291r.634tttcctctccggagga>t1.000
rrl1474356r.699t>c1.000
rrl1474362r.705a>g1.000
rrl1474376r.719t>c1.000
rrl1474639r.982g>a1.000
rrl1474794r.1137c>t1.000
rrl1474812r.1155g>a1.000
rrl1474823r.1166c>t1.000
rrl1474831r.1174a>g1.000
rrl1474904r.1247g>a1.000
rrl1474956r.1299c>t1.000
rrl1474960r.1303c>g1.000
rrl1474962r.1305t>c1.000
rrl1474963r.1306g>t1.000
rrl1474964r.1307t>a1.000
rrl1474965r.1308g>c1.000
rrl1474970r.1313g>a1.000
rrl1475006r.1349a>t1.000
rrl1475013r.1356g>a1.000
rrl1475061r.1404c>t1.000
rrl1475088r.1431a>t1.000
rrl1475199r.1542g>a1.000
rrl1475206r.1549c>t0.900
rrl1475291r.1634a>c1.000
rrl1475313r.1656g>a1.000
rrl1475315r.1658a>t1.000
rrl1475355r.1698c>t1.000
rrl1475369r.1712g>t1.000
rrl1475402r.1745c>t1.000
rrl1475419r.1762c>t1.000
rrl1475436r.1779c>t1.000
rrl1475483r.1826c>t1.000
rrl1475505r.1848g>a1.000
rrl1475526r.1869c>a1.000
rrl1475573r.1916g>a1.000
rrl1475599r.1942a>g1.000
rrl1475608r.1951t>c1.000
rrl1475642r.1985t>c1.000
rrl1475657r.2000a>g1.000
rrl1475659r.2002g>t1.000
rrl1475758r.2101ac>a1.000
rrl1475763r.2106c>t1.000
rrl1475765r.2108ag>a1.000
rrl1475881r.2224t>c1.000
rrl1476131r.2474c>t1.000
rrl1476183r.2526a>c1.000
rrl1476214r.2557g>t1.000
rrl1476215r.2558c>t1.000
rrl1476224r.2567a>g1.000
rrl1476281r.2624t>c1.000
rrl1476628r.2971t>a1.000
rrl1476689r.3032a>t1.000
rrl1476690r.3033c>t1.000
rrl1476693r.3036g>a1.000
rrl1476695r.3038t>a1.000
rrl1476716r.3059a>c1.000
inhA1674636c.435C>G1.000
inhA1674660c.459G>C1.000
rpsA1833589c.48A>T1.000
rpsA1833595c.54T>C1.000
rpsA1833610c.69C>T1.000
rpsA1833661c.120A>G1.000
rpsA1833667c.126C>G1.000
rpsA1833724c.183C>G1.000
rpsA1833727c.186G>C1.000
rpsA1833742c.201A>G1.000
rpsA1833745c.204G>C1.000
rpsA1833748c.207C>G1.000
rpsA1833772c.231C>T1.000
rpsA1833787c.246C>G1.000
rpsA1833790c.249T>C1.000
rpsA1833808c.267G>C1.000
rpsA1833811c.270G>T1.000
rpsA1833835c.294C>T1.000
rpsA1833838c.297G>C1.000
rpsA1833841c.300C>G1.000
rpsA1833847c.306C>G1.000
rpsA1833856c.315A>G1.000
rpsA1833868c.327C>T1.000
rpsA1833874c.333T>C1.000
rpsA1833928c.387G>C1.000
rpsA1833940c.399C>G1.000
rpsA1833971c.430C>T1.000
rpsA1833979c.438T>C1.000
rpsA1834000c.459G>C1.000
rpsA1834021c.480C>T1.000
rpsA1834030c.489C>G1.000
rpsA1834222c.681T>C1.000
rpsA1834249c.708T>C1.000
rpsA1834339c.798C>T1.000
rpsA1834639c.1098T>C1.000
rpsA1834780c.1239A>G1.000
rpsA1834789c.1248T>C1.000
rpsA1834792c.1251G>C1.000
ahpC2726638p.Ala149Val1.000
ahpC2726645c.453C>G1.000
ahpC2726657c.465A>C1.000
ahpC2726669p.Asp159Glu1.000
ahpC2726672c.480G>A1.000
ahpC2726675c.483A>G1.000
ahpC2726681c.489A>C1.000
ahpC2726693c.501C>A1.000
ahpC2726696c.504C>G1.000
ahpC2726717c.525A>G1.000
thyX3067316c.630T>C1.000
thyX3067325c.621T>C1.000
thyX3067349c.597C>G1.000
thyX3067355c.591T>G1.000
thyX3067367c.579C>G1.000
thyX3067391c.555C>G1.000
thyX3067394c.552C>G1.000
thyX3067898p.Asp16Glu1.000
thyA3073953c.519A>G1.000
thyA3073977c.495T>C1.000
thyA3073989c.483A>G1.000
thyA3074001c.471G>C1.000
thyA3074004c.468A>G1.000
embC4241107c.1245G>C1.000
embC4241131c.1269G>A1.000
embC4241140c.1278A>G1.000
embC4241146c.1284C>G1.000
embC4241158c.1296T>G1.000
embC4241161c.1299C>G1.000
embC4241162c.1300T>C1.000
embA4245137c.1905T>C1.000
embA4245153p.Ile641Val1.000
embB4248169c.1656C>G1.000
embB4248172c.1659G>C1.000
embB4248220c.1707A>T1.000
embB4248241c.1728C>T1.000
embB4249469p.Leu986Met1.000
embB4249486c.2973T>G1.000

Analysis pipeline specifications

TBProfiler Version: 2.1.1

AnalysisProgram
Mappingbwa
Variant CallingBCFtools


Log

Drug Details
The estimated accuracy of drug resistance predictions is:

Drug1Sensitivity (%)Specificity (%)
Isoniazid 92.8 (89.9-95.7)100 (100-100)
Rifampicin 96.2 (93.9-98.5)98.1 (96.8-99.4)
Ethambutal 88.7 (83.6-93.8)81.7 (77.6-85.8)
Pyrazinamide70.9 (62.4-79.4)93.9 (90.6-97.2)
Streptomycin87.1 (82.7-91.5)89.7 (86.0-93.4)
Ethionamide 73.6 (66.7-80.5)93.3 (89.6-97.0)
Moxifloxacin60.0 (29.6-90.4)68.7 (52.6-84.8)
Ofloxacin 85.5 (79.1-91.9)94.9 (91.8-98.0)
Amikacin 82.9 (74.4-91.4)98.3 (96.0-100)
Capreomycin 60.7 (50.6-70.8)90.7 (87.2-94.2)
Kanamycin 87.3 (81.3-93.3)93.4 (89.9-96.9)
Para-aminosalisylic acidNot determinedNot determined
Linezolid Not determinedNot determined
Clofazimine Not determinedNot determined
Bedaquiline Not determinedNot determined
Multi drug resistance91.2 (87.8-94.6)98.4 (97.2-99.6)
Extremely drug resistance75.9 (64.5-87.3)98.4 (97.3-99.5)

Lineages
Lineage2LocationStrainRD type
lineage1Indo-OceanicEAIRD239
lineage1.1Indo-OceanicEAI3;EAI4;EAI5;EAI6RD239
lineage1.1.1Indo-OceanicEAI4;EAI5RD239
lineage1.1.1.1Indo-OceanicEAI4;ZERORD239
lineage1.1.2Indo-OceanicEAI3;EAI5RD239
lineage1.1.3Indo-OceanicEAI6RD239
lineage1.2Indo-OceanicEAI1;EAI2RD239
lineage1.2.1Indo-OceanicEAI2RD239
lineage1.2.2Indo-OceanicEAI1RD239
lineage2East-AsianBeijingRD105
lineage2.1East-Asian (non-Beijing)NoneNone
lineage2.2East-Asian (Beijing)Beijing-RD207RD105;RD207
lineage2.2.2East-Asian (Beijing)Beijing-RD105/RD207RD105;RD207
lineage2.2.1East-Asian (Beijing)Beijing-RD181RD105;RD207;RD181
lineage2.2.1.2East-Asian (Beijing)Beijing-RD142RD105;RD207;RD181;RD142
lineage2.2.1.1East-Asian (Beijing)Beijing-RD150RD105;RD207;RD181;RD150
lineage3East-African-IndianCASRD750
lineage3.1East-African-IndianNon-CAS1-DelhiRD750
lineage3.1.1East-African-IndianCAS1-KiliRD750
lineage3.1.2East-African-IndianCAS;CAS2RD750
lineage3.1.2.1East-African-IndianCAS2RD750
lineage3.1.2.2East-African-IndianCASRD750
lineage4Euro-AmericanLAM;T;S;X;HNone
lineage4.1Euro-AmericanT;X;HNone
lineage4.1.2Euro-American (X-type)T;HNone
lineage4.1.2.1Euro-American (X-type)T1;H1RD182
lineage4.1.1Euro-American (X-type)X1;X2;X3None
lineage4.1.1.2Euro-American (X-type)X1None
lineage4.1.1.1Euro-American (X-type)X2RD183
lineage4.1.1.3Euro-American (Haarlem)X1;X3RD193
lineage4.2Euro-AmericanH;T;LAMNone
lineage4.2.2Euro-American (Ural)T;LAM7-TURNone
lineage4.2.2.1Euro-AmericanLAM7-TURNone
lineage4.2.1Euro-American (TUR)H3;H4None
lineage4.3Euro-American (LAM)mainly-LAMNone
lineage4.3.1Euro-American (LAM)LAM9None
lineage4.3.3Euro-American (LAM)LAM;TRD115
lineage4.3.4Euro-American (LAM)LAMRD174
lineage4.3.4.1Euro-American (LAM)LAM1;LAM2RD174
lineage4.3.4.2Euro-American (LAM)LAM1;LAM4;LAM11RD174
lineage4.3.4.2.1Euro-American (LAM)LAM11RD174
lineage4.3.2Euro-American (LAM)LAM3None
lineage4.3.2.1Euro-American (LAM)LAM3RD761
lineage4.4Euro-AmericanS;TNone
lineage4.4.2Euro-AmericanT1;T2None
lineage4.4.1Euro-American (S-type)S;TNone
lineage4.4.1.2Euro-AmericanT1None
lineage4.4.1.1Euro-AmericanS;OrphansNone
lineage4.5Euro-AmericanH;TRD122
lineage4.6Euro-AmericanT;LAMNone
lineage4.6.1Euro-American (Uganda)T2RD724
lineage4.6.1.1Euro-American T2-UgandaRD724
lineage4.6.1.2Euro-AmericanT2RD724
lineage4.6.2Euro-AmericanT;LAMRD726
lineage4.6.2.1Euro-AmericanT3RD726
lineage4.6.2.2Euro-American (Cameroon)LAM10-CAMRD726
lineage4.7Euro-American (mainly T)T1;T5None
lineage4.8Euro-American (mainly T)T1;T2;T3;T5RD219
lineage4.9Euro-American (H37Rv-like)T1None
lineage5West-Africa 1AFRI_2;AFRI_3RD711
lineage6West-Africa 2AFRI_1RD702
lineage7Lineage 7NoneNone
lineageBOVM. bovisBOV_1;BOV_2None

Citations
1. Coll F, et al. Rapid determination of anti-tuberculosis drug resistance from whole genome sequences. Genome Medicine (in press)
2. Coll F, et al. A robust SNP barcode for typing Mycobacterium tuberculosis complex strains. Nature Communications 2014.

Disclaimer
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