TB-Profiler result

Run: SRR22528528
Summary

Run ID: SRR22528528

Sample name:

Date: 2024-04-14T22:56:05.622599

Number of reads: 1853841

Percentage reads mapped: 99.76

Median coverage: 62.0

Strain: lineage4.4.1.1

Spoligotype:

Drug-resistance: MDR-TB

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Lineage Table: The lineage is inferred by analysing lineage specific SNPs
Lineage Family RDs Frequency
lineage2.2.1 East-Asian (Beijing) RD105;RD207;RD181 0.11
lineage4.4.1 Euro-American (S-type) None 0.87
lineage4 Euro-American None 0.96
lineage4.4.1.1 Euro-American None 0.89
lineage4.4 Euro-American None 0.91
Drug resistance: This table reports drug-resistance associated mutations found in known resistance genes
Drug Resistance Supporting mutations WHO confidence Comment
rifampicin rpoB p.Ser450Leu Assoc w R
isoniazid katG p.Ser315Thr Assoc w R High-level resistance
ethambutol
pyrazinamide
streptomycin rpsL p.Lys43Arg Assoc w R
fluoroquinolones
moxifloxacin
ofloxacin
levofloxacin
ciprofloxacin
aminoglycosides
amikacin
capreomycin
kanamycin
cycloserine
ethionamide
clofazimine
para-aminosalicylic_acid
delamanid
bedaquiline
linezolid
Drug resistance-Associated Mutations: This table reports mutations found in candidate resistance genes which have been associated with drug resistance
Gene Chromosome position Mutation Type Estimated fraction Drugs Confidence Comment
rpoB 761155 p.Ser450Leu missense_variant 0.9 rifampicin Assoc w R
rpsL 781687 p.Lys43Arg missense_variant 0.92 streptomycin Assoc w R
katG 2155168 p.Ser315Thr missense_variant 0.89 isoniazid Assoc w R High-level resistance
Non-Associated Mutations: This table reports mutations found in candidate resistance genes which have not been associated with drug resistance
Gene Chromosome position Mutation Type Estimated fraction Drugs Confidence
gyrB 6289 c.1050C>A synonymous_variant 0.19 levofloxacin Not assoc w R - Interim
moxifloxacin
gyrA 7362 p.Glu21Gln missense_variant 1.0 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
gyrA 7585 p.Ser95Thr missense_variant 1.0 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
gyrA 8116 p.Thr272Ile missense_variant 0.8 levofloxacin Uncertain significance
moxifloxacin Uncertain significance
gyrA 9304 p.Gly668Asp missense_variant 1.0 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
Rv0010c 13634 c.-76G>A upstream_gene_variant 0.94 isoniazid Uncertain significance
Rv0565c 657142 p.Arg110His missense_variant 0.15 ethionamide Uncertain significance
rpoB 761528 p.Asp574Glu missense_variant 0.94 rifampicin Uncertain significance
mmpL5 775639 p.Ile948Val missense_variant 1.0 clofazimine Not assoc w R
bedaquiline Not assoc w R - Interim
mmpL5 776100 p.Thr794Ile missense_variant 0.14 clofazimine Not assoc w R
bedaquiline Not assoc w R - Interim
mmpS5 779615 c.-710C>G upstream_gene_variant 0.2 clofazimine
bedaquiline
rpsL 781395 c.-165T>C upstream_gene_variant 1.0 streptomycin Not assoc w R
rrs 1471659 n.-187C>T upstream_gene_variant 1.0 streptomycin
kanamycin
capreomycin
amikacin
tsnR 1854300 p.Leu232Pro missense_variant 1.0 linezolid Not assoc w R
tlyA 1917972 c.33A>G synonymous_variant 1.0 capreomycin Not assoc w R
ndh 2102990 p.Val18Ala missense_variant 0.96 ethionamide Uncertain significance
delamanid Uncertain significance
isoniazid Not assoc w R
PPE35 2169840 p.Gly258Asp missense_variant 0.83 pyrazinamide Not assoc w R
Rv1979c 2223293 c.-129A>G upstream_gene_variant 1.0 clofazimine Not assoc w R
bedaquiline Not assoc w R - Interim
thyA 3074495 c.-24C>T upstream_gene_variant 0.87 para-aminosalicylic_acid
ald 3086788 c.-32T>C upstream_gene_variant 1.0 cycloserine
Rv3083 3448608 c.105G>A synonymous_variant 0.92 ethionamide Not assoc w R - Interim
whiB7 3569220 c.-541C>T upstream_gene_variant 0.24 streptomycin
amikacin
kanamycin
Rv3236c 3612665 p.Val151Ala missense_variant 0.86 pyrazinamide Not assoc w R
mtrB 3625065 p.Met517Leu missense_variant 0.98 bedaquiline Uncertain significance
rifampicin Not assoc w R
mtrB 3625771 c.843C>T synonymous_variant 0.96 bedaquiline
rifampicin Not assoc w R - Interim
embC 4238963 c.-900C>T upstream_gene_variant 0.94 ethambutol Not assoc w R
embC 4239408 c.-455G>A upstream_gene_variant 0.17 ethambutol Uncertain significance
embC 4242643 c.2781C>T synonymous_variant 1.0 ethambutol Not assoc w R
embA 4244970 c.1738C>T synonymous_variant 0.92 ethambutol Not assoc w R - Interim
embB 4247028 p.Leu172Arg missense_variant 0.21 ethambutol
embB 4248437 p.Thr642Ala missense_variant 0.91 ethambutol Uncertain significance
aftB 4267647 p.Asp397Gly missense_variant 0.14 ethambutol Not assoc w R
ethA 4326676 p.Ser266Arg missense_variant 0.12 ethionamide Uncertain significance
ethA 4326703 c.771A>G synonymous_variant 0.93 ethionamide Not assoc w R - Interim
whiB6 4338514 p.Tyr3Cys missense_variant 0.95 capreomycin Uncertain significance
amikacin Uncertain significance
kanamycin Uncertain significance
whiB6 4338595 c.-75delG upstream_gene_variant 1.0 capreomycin Not assoc w R
amikacin Not assoc w R
kanamycin Not assoc w R
whiB6 4338732 c.-211C>T upstream_gene_variant 1.0 capreomycin
amikacin
kanamycin
gid 4407588 c.615A>G synonymous_variant 0.12 streptomycin Not assoc w R
gid 4407927 p.Glu92Asp missense_variant 0.19 streptomycin Not assoc w R