TB-Profiler result

Run: ERR4024831
Summary

Run ID: ERR4024831

Sample name:

Date: 2024-04-15T21:17:03.028413

Number of reads: 2531920

Percentage reads mapped: 99.66

Median coverage: 77.0

Strain: lineage4.3.3;lineage4.4.1.1

Spoligotype:

Drug-resistance: MDR-TB

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Lineage Table: The lineage is inferred by analysing lineage specific SNPs
Lineage Family RDs Frequency
lineage4.3 Euro-American (LAM) None 0.83
lineage4.4.1 Euro-American (S-type) None 0.14
lineage4 Euro-American None 1.0
lineage4.4.1.1 Euro-American None 0.16
lineage4.3.3 Euro-American (LAM) RD115 0.86
lineage4.4 Euro-American None 0.17
Drug resistance: This table reports drug-resistance associated mutations found in known resistance genes
Drug Resistance Supporting mutations WHO confidence Comment
rifampicin rpoB p.Asp435Val Assoc w R
isoniazid katG p.Ser315Thr Assoc w R High-level resistance
ethambutol embB p.Tyr319Ser Assoc w R
pyrazinamide pncA p.Gln10Arg Assoc w R
streptomycin gid p.Pro84Leu Assoc w R - Interim
fluoroquinolones
moxifloxacin
ofloxacin
levofloxacin
ciprofloxacin
aminoglycosides
amikacin
capreomycin tlyA p.Gly232Asp Assoc w R - Interim
kanamycin
cycloserine
ethionamide
clofazimine
para-aminosalicylic_acid
delamanid
bedaquiline
linezolid
Drug resistance-Associated Mutations: This table reports mutations found in candidate resistance genes which have been associated with drug resistance
Gene Chromosome position Mutation Type Estimated fraction Drugs Confidence Comment
rpoB 761110 p.Asp435Val missense_variant 0.83 rifampicin Assoc w R
tlyA 1918634 p.Gly232Asp missense_variant 0.76 capreomycin Assoc w R - Interim
katG 2155168 p.Ser315Thr missense_variant 0.88 isoniazid Assoc w R High-level resistance
pncA 2289213 p.Gln10Arg missense_variant 0.76 pyrazinamide Assoc w R
embB 4247469 p.Tyr319Ser missense_variant 0.88 ethambutol Assoc w R
gid 4407952 p.Pro84Leu missense_variant 0.87 streptomycin Assoc w R - Interim
Non-Associated Mutations: This table reports mutations found in candidate resistance genes which have not been associated with drug resistance
Gene Chromosome position Mutation Type Estimated fraction Drugs Confidence
gyrA 7362 p.Glu21Gln missense_variant 1.0 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
gyrA 7585 p.Ser95Thr missense_variant 1.0 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
gyrA 8040 p.Gly247Ser missense_variant 0.9 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
gyrA 9304 p.Gly668Asp missense_variant 1.0 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
rpoC 764995 c.1626C>G synonymous_variant 0.85 rifampicin Not assoc w R
mmpL5 775639 p.Ile948Val missense_variant 1.0 clofazimine Not assoc w R
bedaquiline Not assoc w R - Interim
rpsL 781395 c.-165T>C upstream_gene_variant 1.0 streptomycin Not assoc w R
rplC 800692 c.-117C>T upstream_gene_variant 0.16 linezolid
rrs 1471659 n.-187C>T upstream_gene_variant 1.0 streptomycin
kanamycin
capreomycin
amikacin
rpsA 1834985 c.1445dupG frameshift_variant&stop_lost&splice_region_variant 0.88 pyrazinamide Uncertain significance
tsnR 1854300 p.Leu232Pro missense_variant 1.0 linezolid Not assoc w R
tlyA 1917972 c.33A>G synonymous_variant 1.0 capreomycin Not assoc w R
ndh 2102473 c.570C>T synonymous_variant 0.14 ethionamide Not assoc w R - Interim
delamanid Not assoc w R - Interim
isoniazid Not assoc w R - Interim
ndh 2102990 p.Val18Ala missense_variant 0.13 ethionamide Uncertain significance
delamanid Uncertain significance
isoniazid Not assoc w R
PPE35 2168998 p.Ile539Val missense_variant 0.81 pyrazinamide Uncertain significance
PPE35 2169840 p.Gly258Asp missense_variant 0.14 pyrazinamide Not assoc w R
Rv1979c 2223293 c.-129A>G upstream_gene_variant 1.0 clofazimine Not assoc w R
bedaquiline Not assoc w R - Interim
kasA 2518919 p.Gly269Ser missense_variant 0.96 isoniazid
Rv2477c 2783921 p.Gly41Glu missense_variant 0.73 ethambutol Uncertain significance
moxifloxacin Uncertain significance
streptomycin Uncertain significance
levofloxacin Uncertain significance
kanamycin Uncertain significance
rifampicin Not assoc w R
amikacin Uncertain significance
thyX 3068043 c.-98A>G upstream_gene_variant 0.13 para-aminosalicylic_acid
thyA 3073868 p.Thr202Ala missense_variant 0.84 para-aminosalicylic_acid
ald 3086788 c.-32T>C upstream_gene_variant 1.0 cycloserine
Rv3083 3448608 c.105G>A synonymous_variant 0.18 ethionamide Not assoc w R - Interim
Rv3236c 3612665 p.Val151Ala missense_variant 0.17 pyrazinamide Not assoc w R
mtrB 3625065 p.Met517Leu missense_variant 1.0 bedaquiline Uncertain significance
rifampicin Not assoc w R
clpC1 4038287 c.2418C>T synonymous_variant 0.84 pyrazinamide Not assoc w R
embC 4238963 c.-900C>T upstream_gene_variant 0.26 ethambutol Not assoc w R
embC 4242182 p.Ala774Ser missense_variant 0.78 ethambutol Not assoc w R
embC 4242643 c.2781C>T synonymous_variant 1.0 ethambutol Not assoc w R
whiB6 4338595 c.-75delG upstream_gene_variant 1.0 capreomycin Not assoc w R
amikacin Not assoc w R
kanamycin Not assoc w R
whiB6 4338732 c.-211C>T upstream_gene_variant 1.0 capreomycin
amikacin
kanamycin
whiB6 4338744 c.-223T>G upstream_gene_variant 0.2 capreomycin
amikacin
kanamycin
gid 4408156 p.Leu16Arg missense_variant 0.85 streptomycin Not assoc w R