TB-Profiler result

Run: ERR4812981
Summary

Run ID: ERR4812981

Sample name:

Date: 2024-03-25T18:29:09.768850

Number of reads: 2346671

Percentage reads mapped: 99.7

Median coverage: 70.0

Strain: lineage4.3.3;lineage4.8

Spoligotype:

Drug-resistance: MDR-TB

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Lineage Table: The lineage is inferred by analysing lineage specific SNPs
Lineage Family RDs Frequency
lineage4.3 Euro-American (LAM) None 0.31
lineage4 Euro-American None 1.0
lineage4.8 Euro-American (mainly T) RD219 0.69
lineage4.3.3 Euro-American (LAM) RD115 0.28
Drug resistance: This table reports drug-resistance associated mutations found in known resistance genes
Drug Resistance Supporting mutations WHO confidence Comment
rifampicin rpoB p.Asp435Val Assoc w R
isoniazid katG p.Ser315Thr Assoc w R High-level resistance
ethambutol embB p.Tyr319Ser Assoc w R
pyrazinamide pncA p.Gln10Arg Assoc w R
streptomycin gid p.Pro84Leu Assoc w R - Interim
fluoroquinolones
moxifloxacin
ofloxacin
levofloxacin
ciprofloxacin
aminoglycosides
amikacin
capreomycin
kanamycin
cycloserine
ethionamide
clofazimine
para-aminosalicylic_acid
delamanid
bedaquiline
linezolid
Drug resistance-Associated Mutations: This table reports mutations found in candidate resistance genes which have been associated with drug resistance
Gene Chromosome position Mutation Type Estimated fraction Drugs Confidence Comment
rpoB 761110 p.Asp435Val missense_variant 0.25 rifampicin Assoc w R
katG 2155168 p.Ser315Thr missense_variant 0.32 isoniazid Assoc w R High-level resistance
pncA 2289213 p.Gln10Arg missense_variant 0.35 pyrazinamide Assoc w R
embB 4247469 p.Tyr319Ser missense_variant 0.33 ethambutol Assoc w R
gid 4407952 p.Pro84Leu missense_variant 0.33 streptomycin Assoc w R - Interim
Non-Associated Mutations: This table reports mutations found in candidate resistance genes which have not been associated with drug resistance
Gene Chromosome position Mutation Type Estimated fraction Drugs Confidence
dnaA 371 p.Pro124Leu missense_variant 0.54 isoniazid Not assoc w R
dnaA 390 c.390C>T synonymous_variant 0.55 isoniazid Not assoc w R - Interim
dnaA 395 p.Glu132Gly missense_variant 0.56 isoniazid Uncertain significance
gyrB 5077 c.-163_-162insC upstream_gene_variant 0.46 levofloxacin
moxifloxacin
gyrA 7362 p.Glu21Gln missense_variant 1.0 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
gyrA 7585 p.Ser95Thr missense_variant 0.28 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
gyrA 8040 p.Gly247Ser missense_variant 0.31 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
gyrA 9304 p.Gly668Asp missense_variant 0.43 levofloxacin Not assoc w R
moxifloxacin Not assoc w R
rpoC 764995 c.1626C>G synonymous_variant 0.23 rifampicin Not assoc w R
mmpL5 775639 p.Ile948Val missense_variant 1.0 clofazimine Not assoc w R
bedaquiline Not assoc w R - Interim
rpsL 781395 c.-165T>C upstream_gene_variant 1.0 streptomycin Not assoc w R
embR 1417743 c.-396C>T upstream_gene_variant 0.54 ethambutol
rrs 1471659 n.-187C>T upstream_gene_variant 1.0 streptomycin
kanamycin
capreomycin
amikacin
rrl 1474001 n.344C>T non_coding_transcript_exon_variant 0.55 capreomycin Not assoc w R
linezolid Not assoc w R
rpsA 1834985 c.1445dupG frameshift_variant&stop_lost&splice_region_variant 0.42 pyrazinamide Uncertain significance
tsnR 1854300 p.Leu232Pro missense_variant 0.41 linezolid Not assoc w R
tlyA 1917972 c.33A>G synonymous_variant 1.0 capreomycin Not assoc w R
katG 2156384 c.-273G>A upstream_gene_variant 0.64 isoniazid Uncertain significance
PPE35 2168149 p.Pro822Ser missense_variant 0.16 pyrazinamide Not assoc w R
PPE35 2168998 p.Ile539Val missense_variant 0.22 pyrazinamide Uncertain significance
Rv1979c 2223293 c.-129A>G upstream_gene_variant 1.0 clofazimine Not assoc w R
bedaquiline Not assoc w R - Interim
kasA 2518919 p.Gly269Ser missense_variant 0.44 isoniazid
Rv2680 2995848 c.-257G>A upstream_gene_variant 0.13 capreomycin
thyA 3073868 p.Thr202Ala missense_variant 0.24 para-aminosalicylic_acid
ald 3086788 c.-32T>C upstream_gene_variant 0.28 cycloserine
mtrB 3625065 p.Met517Leu missense_variant 0.33 bedaquiline Uncertain significance
rifampicin Not assoc w R
alr 3840764 c.657G>C synonymous_variant 0.57 cycloserine
clpC1 4038287 c.2418C>T synonymous_variant 0.27 pyrazinamide Not assoc w R
embC 4241383 c.1521G>C synonymous_variant 0.62 ethambutol Not assoc w R - Interim
embC 4242182 p.Ala774Ser missense_variant 0.33 ethambutol Not assoc w R
embC 4242643 c.2781C>T synonymous_variant 1.0 ethambutol Not assoc w R
embC 4242946 p.Asn1028Lys missense_variant 0.62 ethambutol Uncertain significance
whiB6 4338595 c.-75delG upstream_gene_variant 1.0 capreomycin Not assoc w R
amikacin Not assoc w R
kanamycin Not assoc w R
whiB6 4338732 c.-211C>T upstream_gene_variant 0.17 capreomycin
amikacin
kanamycin
gid 4408156 p.Leu16Arg missense_variant 0.34 streptomycin Not assoc w R