Run ID: SRR11972306
Sample name:
Date: 2024-10-05T00:51:18.759545
Number of reads: 2317672
Percentage reads mapped: 94.4
Median coverage: 92.0
Strain: lineage2.2.1
Spoligotype:
Drug-resistance: Pre-XDR-TB
| Lineage | Family | RDs | Frequency |
|---|---|---|---|
| lineage2 | East-Asian | RD105 | 1.0 |
| lineage2.2.1 | East-Asian (Beijing) | RD105;RD207;RD181 | 1.0 |
| lineage2.2 | East-Asian (Beijing) | RD105;RD207 | 1.0 |
| Drug | Resistance | Supporting mutations | WHO confidence | Comment |
|---|---|---|---|---|
| rifampicin | rpoB | p.Ser450Leu | Assoc w R | |
| isoniazid | katG | p.Ser315Thr | Assoc w R | High-level resistance |
| ethambutol | embB | p.Met306Ile | Assoc w R | |
| pyrazinamide | pncA | p.Ile6Thr | Assoc w R | |
| streptomycin | rpsL | p.Lys43Arg | Assoc w R | |
| fluoroquinolones | ||||
| moxifloxacin | gyrA | p.Asp94Gly | Assoc w R | High-level resistance |
| ofloxacin | ||||
| levofloxacin | gyrA | p.Asp94Gly | Assoc w R | |
| ciprofloxacin | ||||
| aminoglycosides | ||||
| amikacin | ||||
| capreomycin | ||||
| kanamycin | ||||
| cycloserine | ||||
| ethionamide | ||||
| clofazimine | ||||
| para-aminosalicylic_acid | ||||
| delamanid | ||||
| bedaquiline | ||||
| linezolid |
| Gene | Chromosome position | Mutation | Type | Estimated fraction | Drugs | Confidence | Comment |
|---|---|---|---|---|---|---|---|
| gyrA | 7582 | p.Asp94Gly | missense_variant | 0.91 | levofloxacin | Assoc w R | |
| moxifloxacin | Assoc w R | High-level resistance | |||||
| rpoB | 761155 | p.Ser450Leu | missense_variant | 0.89 | rifampicin | Assoc w R | |
| rpsL | 781687 | p.Lys43Arg | missense_variant | 1.0 | streptomycin | Assoc w R | |
| katG | 2155168 | p.Ser315Thr | missense_variant | 0.98 | isoniazid | Assoc w R | High-level resistance |
| pncA | 2289225 | p.Ile6Thr | missense_variant | 1.0 | pyrazinamide | Assoc w R | |
| embB | 4247431 | p.Met306Ile | missense_variant | 0.98 | ethambutol | Assoc w R |
| Gene | Chromosome position | Mutation | Type | Estimated fraction | Drugs | Confidence |
|---|---|---|---|---|---|---|
| gyrB | 4969 | c.-271G>T | upstream_gene_variant | 1.0 | levofloxacin | |
| moxifloxacin | ||||||
| gyrA | 7362 | p.Glu21Gln | missense_variant | 1.0 | levofloxacin | Not assoc w R |
| moxifloxacin | Not assoc w R | |||||
| gyrA | 7585 | p.Ser95Thr | missense_variant | 1.0 | levofloxacin | Not assoc w R |
| moxifloxacin | Not assoc w R | |||||
| gyrA | 9304 | p.Gly668Asp | missense_variant | 1.0 | levofloxacin | Not assoc w R |
| moxifloxacin | Not assoc w R | |||||
| fgd1 | 491742 | c.960T>C | synonymous_variant | 1.0 | pretomanid | |
| delamanid | Not assoc w R - Interim | |||||
| clofazimine | Not assoc w R | |||||
| mshA | 575907 | p.Ala187Val | missense_variant | 0.99 | isoniazid | Not assoc w R |
| ethionamide | Not assoc w R | |||||
| ccsA | 620625 | p.Ile245Met | missense_variant | 1.0 | kanamycin | Uncertain significance |
| capreomycin | Not assoc w R | |||||
| amikacin | Not assoc w R | |||||
| Rv0565c | 657081 | c.390G>A | synonymous_variant | 1.0 | ethionamide | Not assoc w R - Interim |
| Rv0565c | 657142 | p.Arg110His | missense_variant | 1.0 | ethionamide | Uncertain significance |
| rpoB | 760591 | p.Val262Ala | missense_variant | 0.14 | rifampicin | |
| rpoB | 760611 | c.805T>C | synonymous_variant | 0.14 | rifampicin | Not assoc w R - Interim |
| rpoB | 762176 | c.2370T>C | synonymous_variant | 0.13 | rifampicin | Not assoc w R - Interim |
| rpoB | 763031 | c.3225T>C | synonymous_variant | 1.0 | rifampicin | Not assoc w R |
| rpoC | 764058 | p.Ala230Val | missense_variant | 0.93 | rifampicin | Uncertain significance |
| rpoC | 764635 | c.1266C>G | synonymous_variant | 0.14 | rifampicin | |
| rpoC | 764650 | c.1281G>T | synonymous_variant | 0.14 | rifampicin | Not assoc w R - Interim |
| rpoC | 765370 | c.2001G>C | synonymous_variant | 0.14 | rifampicin | Not assoc w R - Interim |
| rpoC | 765462 | p.Asn698Ser | missense_variant | 0.9 | rifampicin | Uncertain significance |
| rpoC | 765700 | c.2331T>C | synonymous_variant | 0.13 | rifampicin | Not assoc w R - Interim |
| rpoC | 765835 | c.2466C>T | synonymous_variant | 0.13 | rifampicin | Not assoc w R - Interim |
| mmpL5 | 775639 | p.Ile948Val | missense_variant | 1.0 | bedaquiline | Not assoc w R - Interim |
| clofazimine | Not assoc w R | |||||
| mmpL5 | 776100 | p.Thr794Ile | missense_variant | 1.0 | bedaquiline | Not assoc w R - Interim |
| clofazimine | Not assoc w R | |||||
| mmpL5 | 776182 | p.Asp767Asn | missense_variant | 1.0 | bedaquiline | Not assoc w R |
| clofazimine | Not assoc w R | |||||
| mmpS5 | 779615 | c.-710C>G | upstream_gene_variant | 1.0 | bedaquiline | |
| clofazimine | ||||||
| rpsL | 781395 | c.-165T>C | upstream_gene_variant | 1.0 | streptomycin | Not assoc w R |
| Rv1129c | 1254562 | c.-28T>C | upstream_gene_variant | 1.0 | levofloxacin | Not assoc w R |
| moxifloxacin | Not assoc w R | |||||
| isoniazid | Not assoc w R | |||||
| rifampicin | Not assoc w R | |||||
| Rv1258c | 1406760 | c.580_581insC | frameshift_variant | 1.0 | streptomycin | Uncertain significance |
| pyrazinamide | Uncertain significance | |||||
| isoniazid | Uncertain significance | |||||
| rrs | 1471659 | n.-187C>T | upstream_gene_variant | 1.0 | kanamycin | |
| capreomycin | ||||||
| streptomycin | ||||||
| amikacin | ||||||
| rrl | 1474054 | n.397T>C | non_coding_transcript_exon_variant | 0.12 | capreomycin | Uncertain significance |
| linezolid | ||||||
| rrl | 1474093 | n.436G>T | non_coding_transcript_exon_variant | 0.14 | capreomycin | |
| linezolid | ||||||
| rrl | 1474099 | n.442G>A | non_coding_transcript_exon_variant | 0.12 | capreomycin | |
| linezolid | ||||||
| rrl | 1474103 | n.446A>T | non_coding_transcript_exon_variant | 0.12 | capreomycin | |
| linezolid | ||||||
| rrl | 1474181 | n.524C>T | non_coding_transcript_exon_variant | 0.12 | capreomycin | Uncertain significance |
| linezolid | ||||||
| rrl | 1475199 | n.1542G>A | non_coding_transcript_exon_variant | 0.16 | capreomycin | |
| linezolid | ||||||
| rrl | 1475201 | n.1544G>A | non_coding_transcript_exon_variant | 0.17 | capreomycin | |
| linezolid | ||||||
| rrl | 1475206 | n.1549C>T | non_coding_transcript_exon_variant | 0.17 | capreomycin | Uncertain significance |
| linezolid | ||||||
| rrl | 1475209 | n.1552G>C | non_coding_transcript_exon_variant | 0.17 | capreomycin | Uncertain significance |
| linezolid | ||||||
| rrl | 1475213 | n.1556C>T | non_coding_transcript_exon_variant | 0.17 | capreomycin | Uncertain significance |
| linezolid | ||||||
| rrl | 1475249 | n.1592T>C | non_coding_transcript_exon_variant | 0.19 | capreomycin | Uncertain significance |
| linezolid | Uncertain significance | |||||
| rrl | 1475252 | n.1595C>T | non_coding_transcript_exon_variant | 0.19 | capreomycin | |
| linezolid | ||||||
| rrl | 1475266 | n.1609T>C | non_coding_transcript_exon_variant | 0.17 | capreomycin | Uncertain significance |
| linezolid | Uncertain significance | |||||
| rrl | 1475267 | n.1610G>A | non_coding_transcript_exon_variant | 0.16 | capreomycin | |
| linezolid | ||||||
| rrl | 1475275 | n.1618C>T | non_coding_transcript_exon_variant | 0.16 | capreomycin | |
| linezolid | ||||||
| rrl | 1475276 | n.1619T>C | non_coding_transcript_exon_variant | 0.16 | capreomycin | |
| linezolid | ||||||
| rrl | 1475291 | n.1634A>C | non_coding_transcript_exon_variant | 0.16 | capreomycin | Uncertain significance |
| linezolid | Uncertain significance | |||||
| rrl | 1475315 | n.1658A>T | non_coding_transcript_exon_variant | 0.14 | capreomycin | Uncertain significance |
| linezolid | Uncertain significance | |||||
| rrl | 1475369 | n.1712G>C | non_coding_transcript_exon_variant | 0.13 | capreomycin | |
| linezolid | ||||||
| rrl | 1475409 | n.1752T>C | non_coding_transcript_exon_variant | 0.13 | capreomycin | |
| linezolid | ||||||
| rrl | 1475483 | n.1826C>T | non_coding_transcript_exon_variant | 0.15 | capreomycin | Uncertain significance |
| linezolid | Uncertain significance | |||||
| rrl | 1475505 | n.1848G>A | non_coding_transcript_exon_variant | 0.12 | capreomycin | Uncertain significance |
| linezolid | ||||||
| rrl | 1475526 | n.1869C>A | non_coding_transcript_exon_variant | 0.13 | capreomycin | Uncertain significance |
| linezolid | Uncertain significance | |||||
| rrl | 1475599 | n.1942A>G | non_coding_transcript_exon_variant | 0.13 | capreomycin | |
| linezolid | Uncertain significance | |||||
| rpsA | 1834177 | c.636A>C | synonymous_variant | 1.0 | pyrazinamide | Not assoc w R - Interim |
| tsnR | 1854300 | p.Leu232Pro | missense_variant | 1.0 | linezolid | Not assoc w R |
| tlyA | 1917972 | c.33A>G | synonymous_variant | 1.0 | capreomycin | Not assoc w R |
| katG | 2154724 | p.Arg463Leu | missense_variant | 1.0 | isoniazid | Not assoc w R |
| PPE35 | 2167926 | p.Leu896Ser | missense_variant | 1.0 | pyrazinamide | Not assoc w R |
| Rv1979c | 2223293 | c.-129A>G | upstream_gene_variant | 1.0 | bedaquiline | Not assoc w R - Interim |
| clofazimine | Not assoc w R | |||||
| folC | 2747437 | p.Met54Ile | missense_variant | 0.97 | para-aminosalicylic_acid | |
| Rv2477c | 2783077 | c.966T>C | synonymous_variant | 0.15 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783092 | c.951A>G | synonymous_variant | 0.12 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | Not assoc w R - Interim | |||||
| ethambutol | Not assoc w R - Interim | |||||
| amikacin | ||||||
| Rv2477c | 2783095 | c.948C>G | synonymous_variant | 0.13 | kanamycin | Not assoc w R - Interim |
| levofloxacin | Not assoc w R - Interim | |||||
| moxifloxacin | Not assoc w R - Interim | |||||
| streptomycin | Not assoc w R - Interim | |||||
| rifampicin | Not assoc w R | |||||
| ethambutol | Not assoc w R - Interim | |||||
| amikacin | Not assoc w R - Interim | |||||
| Rv2477c | 2783098 | c.945C>G | synonymous_variant | 0.13 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783101 | c.942G>T | synonymous_variant | 0.13 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783116 | c.927T>C | synonymous_variant | 0.15 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783140 | c.903C>G | synonymous_variant | 0.14 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783149 | c.894G>C | synonymous_variant | 0.18 | kanamycin | Not assoc w R - Interim |
| levofloxacin | Not assoc w R - Interim | |||||
| moxifloxacin | Not assoc w R - Interim | |||||
| streptomycin | ||||||
| rifampicin | Not assoc w R - Interim | |||||
| ethambutol | ||||||
| amikacin | Not assoc w R - Interim | |||||
| Rv2477c | 2783158 | c.885A>C | synonymous_variant | 0.18 | kanamycin | Not assoc w R - Interim |
| levofloxacin | Not assoc w R - Interim | |||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | Not assoc w R - Interim | |||||
| ethambutol | Not assoc w R - Interim | |||||
| amikacin | Not assoc w R - Interim | |||||
| Rv2477c | 2783182 | c.861C>G | synonymous_variant | 0.17 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783185 | c.858G>C | synonymous_variant | 0.18 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783194 | c.849A>G | synonymous_variant | 0.19 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783200 | c.843G>A | synonymous_variant | 0.18 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783620 | c.423C>G | synonymous_variant | 0.13 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783788 | c.255T>C | synonymous_variant | 0.13 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783815 | c.228A>G | synonymous_variant | 0.18 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783821 | p.Gln74Leu | missense_variant | 0.2 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783833 | c.210G>C | synonymous_variant | 0.26 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783839 | c.204C>G | synonymous_variant | 0.24 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783843 | p.Gly67Asp | missense_variant | 0.24 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783846 | p.Thr66Asn | missense_variant | 0.23 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783860 | c.183T>C | synonymous_variant | 0.17 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783883 | c.160T>C | synonymous_variant | 0.15 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783884 | c.159T>C | synonymous_variant | 0.15 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783914 | c.129T>C | synonymous_variant | 0.14 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783920 | c.123G>C | synonymous_variant | 0.14 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| Rv2477c | 2783929 | c.114C>G | synonymous_variant | 0.13 | kanamycin | |
| levofloxacin | ||||||
| moxifloxacin | ||||||
| streptomycin | ||||||
| rifampicin | ||||||
| ethambutol | ||||||
| amikacin | ||||||
| thyX | 3067478 | c.468C>T | synonymous_variant | 0.14 | para-aminosalicylic_acid | |
| thyX | 3067499 | c.447G>A | synonymous_variant | 0.13 | para-aminosalicylic_acid | |
| thyX | 3067504 | p.Ala148Thr | missense_variant | 0.13 | para-aminosalicylic_acid | |
| thyX | 3067515 | p.Ser144Thr | missense_variant | 0.13 | para-aminosalicylic_acid | |
| ald | 3086788 | c.-32T>C | upstream_gene_variant | 1.0 | cycloserine | |
| whiB7 | 3568893 | c.-215delC | upstream_gene_variant | 1.0 | kanamycin | Uncertain significance |
| amikacin | Uncertain significance | |||||
| streptomycin | Uncertain significance | |||||
| Rv3236c | 3612813 | p.Thr102Ala | missense_variant | 1.0 | pyrazinamide | Not assoc w R - Interim |
| mtrB | 3625065 | p.Met517Leu | missense_variant | 1.0 | rifampicin | Not assoc w R |
| bedaquiline | Uncertain significance | |||||
| mtrB | 3626562 | p.Pro18Ser | missense_variant | 1.0 | rifampicin | Not assoc w R |
| bedaquiline | Uncertain significance | |||||
| mtrB | 3626972 | c.-359A>G | upstream_gene_variant | 0.15 | rifampicin | |
| bedaquiline | ||||||
| mtrB | 3626987 | c.-374C>G | upstream_gene_variant | 0.19 | rifampicin | |
| bedaquiline | ||||||
| mtrB | 3627011 | c.-398T>G | upstream_gene_variant | 0.21 | rifampicin | |
| bedaquiline | ||||||
| mtrA | 3627026 | c.324G>A | synonymous_variant | 0.16 | rifampicin | Not assoc w R - Interim |
| bedaquiline | Not assoc w R - Interim | |||||
| rpoA | 3878271 | c.237T>C | synonymous_variant | 0.12 | rifampicin | Not assoc w R - Interim |
| rpoA | 3878337 | c.171T>C | synonymous_variant | 0.13 | rifampicin | Not assoc w R - Interim |
| rpoA | 3878343 | c.165C>G | synonymous_variant | 0.14 | rifampicin | |
| rpoA | 3878346 | c.162T>C | synonymous_variant | 0.14 | rifampicin | |
| rpoA | 3878364 | c.144A>T | synonymous_variant | 0.13 | rifampicin | |
| embC | 4241958 | p.Arg699Leu | missense_variant | 1.0 | ethambutol | Uncertain significance |
| embC | 4242643 | c.2781C>T | synonymous_variant | 0.94 | ethambutol | Not assoc w R |
| embA | 4243460 | c.228C>T | synonymous_variant | 1.0 | ethambutol | Not assoc w R |
| aftB | 4267647 | p.Asp397Gly | missense_variant | 1.0 | ethambutol | Not assoc w R |
| ubiA | 4269310 | p.Trp175Ser | missense_variant | 1.0 | ethambutol | Uncertain significance |
| whiB6 | 4338425 | p.Val33Ile | missense_variant | 1.0 | kanamycin | |
| capreomycin | ||||||
| amikacin | ||||||
| whiB6 | 4338595 | c.-75delG | upstream_gene_variant | 1.0 | kanamycin | Not assoc w R |
| capreomycin | Not assoc w R | |||||
| amikacin | Not assoc w R | |||||
| whiB6 | 4338732 | c.-211C>T | upstream_gene_variant | 1.0 | kanamycin | |
| capreomycin | ||||||
| amikacin | ||||||
| gid | 4407588 | c.615A>G | synonymous_variant | 1.0 | streptomycin | Not assoc w R |
| gid | 4407927 | p.Glu92Asp | missense_variant | 1.0 | streptomycin | Not assoc w R |
